Homogeneous preparation of human thymidine-5'-triphosphatase by electroelution from SDS/PAGE with subsequent renaturation
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چکیده
منابع مشابه
Preparation of 4'-substituted thymidines by substitution of the thymidine 5'-esters.
tert-Butyl thymidylate 3 was prepared from thymidine 1 in six steps and 67% overall yield. When the lithium trianion of 3 (prepared by treatment of 3 with excess LDA and then excess tert-butyllithum) is reacted with electrophiles, trapping occurs stereoselectively from either the alpha- or beta-face depending on the electrophile (Scheme 1). Deuterioacetic acid in deuteriomethanol affords mainly...
متن کاملThe preparation of homogeneous monkey and human pituitary growth hormones.
Simian pituitary growth hormone was prepared in homogeneous form by sequential application of exclusion chromatography on Sephadex G-100, ion exchange chromatography on diethylaminoethyl Sephadex with a single eluent, and density gradient column electrophoresis. DEAESephadex chromatography with a single eluent was also used to separate two biologically active components of human pituitary growt...
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1. Extracts of Landschutz ascites-tumour cells have been fractionated by treatment with acid, alumina C(gamma) gel and Sephadex G-100 to yield purified preparations of thymidine phosphokinase, thymidine 5'-monophosphate phosphokinase and thymidine 5'-diphosphate phosphokinase. 2. These results clearly demonstrate the existence in Landschutz ascites tumour of three phosphokinases each of which c...
متن کاملPreparation and evaluation of 99mTc-labeled zidovudine function as an analog of thymidine in the diagnosis of human lung cancer cells
Introduction: Labeled nucleotide detectors provide a basis for measuring cell division. Zidovudine is a prodrug that is phosphorylated by cellular enzymes at the 5ʹ position and converted to active triphosphate. Zidovudine triphosphate interferes with the reverse transcriptase enzyme and lengthens the viral DNA strand. Materials and Methods: Ethyl-2-propynyl acetate and 2-amino-N-propynyl benza...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1990
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1990.tb19215.x